Journal: Biomedicines

Article Title: miR-195-5p Regulates Tight Junctions Expression via Claudin-2 Downregulation in Ulcerative Colitis

doi: 10.3390/biomedicines10040919

Figure Lengend Snippet: Validation of differentially expressed miRNAs. Expression levels of miR-195a-5p, miR-497a-5p, miR-99a-5p, and miR-199a-3p on miRNAs isolated from colonic IECs. miRNAs expression levels were quantified using qRT-PCR and the relative expression was normalized to the expression of endogenous control miR-186-5p. All tested miRNAs showed a significant change in expression, confirming microarray data. * p < 0.05; ** p < 0.01.

Article Snippet: The labelled RNA was hybridized, washed, stained, and scanned with an Agilent microarray scanner (G2565BA, Agilent).

Techniques: Expressing, Isolation, Quantitative RT-PCR, Microarray

Journal: Biomedicines

Article Title: miR-195-5p Regulates Tight Junctions Expression via Claudin-2 Downregulation in Ulcerative Colitis

doi: 10.3390/biomedicines10040919

Figure Lengend Snippet: miR-195a-5p targets CLDN2. ( A ) Sequence alignment of the miR-195a-5p base-pairing sites in the 3’-UTR of Cldn2 mRNA showing that the regions complementary to miR-195a-5p are highly conserved among mouse, human, and chimp. The “seed” sequences of miR-195a-5p complementary to Cldn2 are shown in white. ( B ) Cldn2 mRNA expression levels evaluated in the same set of RNA samples used in the microarray validation. Cldn2 levels were significantly higher in Winnie mice compared to wild-type mice. Cldn2 expression levels were normalized on the housekeeping gene Gapdh. The histograms represent the mean ± SEM. * p = 0.03. (C) Linear correlation between the expression of Cldn2 and the expression of miR-195a-5p. Cldn2 mRNA levels inversely correlated with miR-195a-5p expression levels. Black points represent correlation between mRNA and miRNA expression for each sample (r = −0.64; p = 0.04).

Article Snippet: The labelled RNA was hybridized, washed, stained, and scanned with an Agilent microarray scanner (G2565BA, Agilent).

Techniques: Sequencing, Expressing, Microarray

Journal: Oncotarget

Article Title: Stromal TGF-β signaling induces AR activation in prostate cancer

doi:

Figure Lengend Snippet: Most up-regulated genes in LNCaP-TGF-β1(a)/HPS19I co-cultures (microarray)

Article Snippet: After hybridization, slides were washed in Gene Expression Wash Buffer 1 and 2 for 1 min and than dried by centrifugation at 2000 rpm for 2 min. Microarrays were scanned with a dynamic autofocus microarray scanner (Agilent Microarray Scanner- G2565BA, Agilent Technologies,) using Agilent-provided parameters (Red and Green PMT were each set at 100%, and scan resolution was set to 5 um).

Techniques: Microarray

Journal: Oncotarget

Article Title: Stromal TGF-β signaling induces AR activation in prostate cancer

doi:

Figure Lengend Snippet: Most down-regulated genes in LNCaP-TGF-β1(a)/HPS19I co-cultures (microarray)

Article Snippet: After hybridization, slides were washed in Gene Expression Wash Buffer 1 and 2 for 1 min and than dried by centrifugation at 2000 rpm for 2 min. Microarrays were scanned with a dynamic autofocus microarray scanner (Agilent Microarray Scanner- G2565BA, Agilent Technologies,) using Agilent-provided parameters (Red and Green PMT were each set at 100%, and scan resolution was set to 5 um).

Techniques: Microarray

Journal: Oncotarget

Article Title: Stromal TGF-β signaling induces AR activation in prostate cancer

doi:

Figure Lengend Snippet: Most up-regulated genes in LNCaP-TGF-β1(a) cell conditioned media treated HPS19I cells (microarray)

Article Snippet: After hybridization, slides were washed in Gene Expression Wash Buffer 1 and 2 for 1 min and than dried by centrifugation at 2000 rpm for 2 min. Microarrays were scanned with a dynamic autofocus microarray scanner (Agilent Microarray Scanner- G2565BA, Agilent Technologies,) using Agilent-provided parameters (Red and Green PMT were each set at 100%, and scan resolution was set to 5 um).

Techniques: Microarray

Journal: Oncotarget

Article Title: Stromal TGF-β signaling induces AR activation in prostate cancer

doi:

Figure Lengend Snippet: Most down-regulated genes in LNCaP-TGF-β1(a) cell conditioned media treated HPS19I cells (microarray)

Article Snippet: After hybridization, slides were washed in Gene Expression Wash Buffer 1 and 2 for 1 min and than dried by centrifugation at 2000 rpm for 2 min. Microarrays were scanned with a dynamic autofocus microarray scanner (Agilent Microarray Scanner- G2565BA, Agilent Technologies,) using Agilent-provided parameters (Red and Green PMT were each set at 100%, and scan resolution was set to 5 um).

Techniques: Microarray